PEmax mRNA ( m1ψ substitution )

PEmax represents a significant architectural optimization of the PE2 system. By incorporating an additional Nuclear Localization Signal (NLS) and a refined nCas9 domain, it achieves superior nuclear import and targeting efficiency. Unlike traditional CRISPR methods, PEmax enables precise genomic modifications without inducing double-stranded breaks (DSBs), thereby maximizing editing yield and broadening the scope of targetable base conversions with enhanced fidelity. Synthesized via in vitro transcription with a Cap 1 structure and poly(A) tail, it mimics mature mRNA to enhance stability, translation efficiency, and performance while minimizing immune responses.

mRNA Length:
9289 nt

Base Composition:
N1-Me-pUTP (N1-mψ)

Concentration:
1.0 mg/ mL

Cap Modification:
Cap 1 structure

Poly A Tail:
Yes

Form:
Liquid

Buffer:
1 mM sodium citrate buffer, pH 6.4.

Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.

Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.

Application:
Gene editing

Shipping Conditions:
Dry ice