PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Thr638. AA range:606-655
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
76kD
GeneID（Human）:
5578
Human Swiss-Prot No:
P17252
Cellular localization:
Cytoplasm . Cell membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Nucleus .
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2
Western Blot analysis of 3T3 cells using Phospho-PKC α (T638) Polyclonal Antibody
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC alpha (Phospho-Thr638) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC alpha (Phospho-Thr638) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using PKC alpha (Phospho-Thr638) Antibody. The lane on the right is blocked with the phospho peptide.