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SKU(재고 관리 코드):PAA458Ge01

Polyclonal Antibody to Testosterone (Testo)

Polyclonal Antibody to Testosterone (Testo)

Organism species
Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.


Organism Species

Pan-species (General).


Polyclonal antibody preparation





Ig Type



Antigen-specific affinity chromatography followed by Protein A affinity chromatography




CPA458Ge11-BSA Conjugated Testosterone (Testo)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.





Organism Species More



ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).

If the antibody is used in flow cytometry, please check FCM antibodies.


20µl 100µl 200µl 1ml 10ml


The antibody is a rabbit polyclonal antibody raised against Testo. It has been selected for its ability to recognize Testo in ELISA and CLIA.


Enzyme-Linked Immune Sorbent Assay: 45ng/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.


Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.


The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
The Tohoku Journal of Experimental Medicine Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats Jstage: source
Plos one Insulin Receptor Substrate 2 Is Required for Testicular Development PubMed: PMC3669358
International Journal of Molecular and Cellular Medicine Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777
Fertility and Sterility Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
Urologia internationalis Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982
International Braz J Urol The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats. Pubmed: 24642156
Journal of Materials Chemistry B Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source
Biochemical and Biophysical Research Communications Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis ScienceDirect: S0006291X14001843
PLoS One. Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate Pubmed:Pmc4100933
Reproductive Toxicology Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190
Physiology & Behavior The impact of maternal consumption of cafeteria diet on reproductive function in the offspring Pubmed:24631302
Int Braz J Urol. The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in rats Pubmed:24642156
Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141
Acta Cir Bras. Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model. Pubmed:25185055
Genet Mol Biol. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846
Turkish Journal of Urology Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831
Int Braz J Urol Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats PubMed: 26200553
Advances in Environmental Biology Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic Extract Aensiweb: Aeb
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886
Environ Toxicol Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period PubMed: 25782374
J Endocrinol Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction PubMed: 25770118
Acta Pharmaceutica Sinica B Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh science:S2211383516301812
Plos One Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642
Theriogenology Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count Pubmed:26948295
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα Pubmed:27413362
Int J Mol Sci. Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
Journal of Applied Life Sciences International Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats Akindele922016JALSI29782.pdf
Integrative Zoology Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management. pubmed:27611741
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362
Andrologia Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943
Turkish journal of medical sciences The effect of kisspeptin on spermatogenesis and apoptosis in rats. pubmed:28263511
Kafkas Universitesi Veteriner Fakultesi Dergisi The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice. 13006045
Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium S2405580816300991
Reproductive Sciences Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome pubmed:28673204
Biomedicine & Pharmacotherapy Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male rats pubmed:28938525
Molecular and Cellular Endocrinology Testosterone deficiency prevents left ventricular contractility dysfunction after myocardial infarction pubmed:28606867
Ankara Üniv Vet Fak Derg The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged mice DOI: 10.1501/Vetfak_0000002791
Acta histochemica Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-) pubmed:28506466
DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625
Aquatic Toxicology Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations Pubmed:29775925
Molecular Reproduction and Development  deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and … Pubmed:29411926
Journal of Ovarian Research DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035
Medical Science Monitor Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … Pubmed:29627845
Oncology Letters Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes … 10.3892:ol.2018.9165
Biomedicine & Pharmacotherapy Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135
Asian Journal of Andrology Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydrates Pubmed: 30198494
第三军医大学学报 基因敲除损伤小鼠睾丸睾酮产生与生精功能
Stem Cells Translational Medicine Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Pubmed: 30569668
Brazilian Journal of Pharmaceutical Science Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146
Journal of Cellular Physiology Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells Pubmed: 30628054
Experimental and Therapeutic Medicine Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
Evidence-Based Complementary and Alternative Medicine iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
International Journal of Reproductive Biomedicine The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.
Kafkas Universitesi Veteriner Fakultesi Dergisi The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice
Journal of Cellular and Molecular Medicine Cyclophilin D participates in the inhibitory effect of high‐fat diet on the expression of steroidogenic acute regulatory protein Pubmed: 31373170
Journal of neuroscience research Revisiting the relationships of 2D:4D with androgen receptor (AR) gene and current testosterone levels: Replication study and meta‐analyses Pubmed: 31359506
Andrologia Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats Pubmed: 31353623
Chemosphere Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938
science of the total environment Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells Pubmed: 32334352
JOURNAL OF MOLECULAR NEUROSCIENCE Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment Pubmed: 33159671
BIOLOGICAL RESEARCH Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance … Pubmed: 33109277
J Food Biochem Novel antihypertensive action of rutin is mediated via inhibition of angiotensin converting enzyme/mineralocorticoid receptor/angiotensin 2 type 1 receptor (ATR1) … Pubmed: 33089540
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio) Pubmed: 33011509
ARCHIVES OF GYNECOLOGY AND OBSTETRICS MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Pubmed: 32757043
Mol Med Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic … Pubmed: 32698821
Antioxidants (Basel) Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model Pubmed: 32517356
Andrologia Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty Pubmed: 32478927
chemosphere N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats Pubmed: 32447106
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488
Iranian Journal of Basic Medical Sciences Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
The effects of gold nanoparticles on Leydig cells and male reproductive function in mice
The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance
JOURNAL OF ETHNOPHARMACOLOGY BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose 33508368
Toxicol Appl Pharmacol Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion 33965371
African Journal of Traditional, Complementary and Alternative Medicines A comparative study on the aphrodisiac activity of food plants< i> Mondia whitei, Chenopodium album, Ccucurbita pe...
Bioengineered MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1 34637688
J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice 34730391
Eur J Pharmacol Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 2 34265299
Theriogenology Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with … Pubmed:35063921
Reprod Sci Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice Pubmed:35257354
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA458Ge11 BSA Conjugated Testosterone (Testo) Immunogen; SDS-PAGE; WB.
CPA458Ge21 OVA Conjugated Testosterone (Testo) Immunogen; SDS-PAGE; WB.
PAA458Ge01 Polyclonal Antibody to Testosterone (Testo) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA458Ge71 Biotin-Linked Polyclonal Antibody to Testosterone (Testo) WB; IHC; ICC.
MAA458Ge27 Monoclonal Antibody to Testosterone (Testo) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA458Ge22 Monoclonal Antibody to Testosterone (Testo) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA458Ge23 Monoclonal Antibody to Testosterone (Testo) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA458Ge24 Monoclonal Antibody to Testosterone (Testo) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA458Ge25 Monoclonal Antibody to Testosterone (Testo) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA458Ge21 Monoclonal Antibody to Testosterone (Testo) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MEA458Ge Mini Samples ELISA Kit for Testosterone (Testo) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA458Ge Instant ELISA Kit for Testosterone (Testo) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA458Ge High Sensitive ELISA Kit for Testosterone (Testo) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA458Ge ELISA Kit for Testosterone (Testo) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA458Ge CLIA Kit for Testosterone (Testo) Chemiluminescent immunoassay for Antigen Detection.
LMA458Ge Magnetic Luminex Assay Kit for Testosterone (Testo) ,etc. Magnetic Luminex Assay for Antigen Detection.
KSA458Ge11 ELISA Kit DIY Materials for Testosterone (Testo) Main materials for "Do It (ELISA Kit) Yourself".
KSA458Ge12 ELISA Kit DIY Materials for Testosterone (Testo) Main materials for "Do It (ELISA Kit) Yourself".

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