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Cloud-clone
SKU(재고 관리 코드):PAA133Bo01
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Product No.
PAA133Bo01
Organism Species
Bos taurus; Bovine (Cattle).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA133Bo01-Recombinant Tumor Necrosis Factor Alpha (TNFa)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Experimental Animals | Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction Jstage: source |
| Not available | Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia Universi: 49434630 |
| 41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. PubMed: 20522592 |
| Respiration | Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice Karger: 000319151 |
| Journal of Huazhong University of Science and Technology-Medical Sciences | Expression and significance of fgl2 prothrombinase in cardiac microvascular endothelial cells of rats with type 2 diabetes SpringerLink: 161m725t24x85kvv |
| The European Journal of Clinical Investigation | Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes Wiley: source |
| Journal of Biomedical Research | Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy ScienceDirect: S1674830111600158 |
| Stem Cells | Combined Effects of Hematopoietic Progenitor Cell Mobilization from Bone Marrow by Granulocyte Colony Stimulating Factor and AMD3100 and Chemotaxis into the Brain Using Stromal Cell-Derived Factor-1α in an Alzheimer's Disease Mouse Model PubMed: 21608078 |
| Journal of Physiology and Biochemistry | Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats SpringerLink: h875w73171w2147w |
| The EMBO Journal | Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice Nature: 2011277 |
| Reproductive BioMedicine Online | Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages ScienceDirect: S1472648312001484 |
| Journal of Vascular and Interventional Radiology | Hepatocellular Necrosis, Apoptosis, and Proliferation after Transcatheter Arterial Embolization or Chemoembolization in a Standardized Rabbit Model PubMed: 21959058 |
| Bone | Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration PubMed: 22796416 |
| Journal of Periodontology | The Effects of a Novel Botanical Agent on LPS-Induced Alveolar Bone Loss in Rats PubMed: 23106504 |
| Ann Rheum Dis. | The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats PubMed: 23172753 |
| Journal of Surgical Research | Enhanced tumor growth after portal vein embolization in a rabbit tumor model PubMed: 23149224 |
| Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process PubMed: 22984252 |
| Journal of Periodontology Online | The Effect of Radachlorin-Mediated Antimicrobial Photodynamic Therapy on Clinical Parameters and Cytokine Profile in Ligature-Induced Periodontitis in Dogs PubMed: 22813345 |
| PLoS One | New Method to Isolate and Culture Rat Kupffer Cells PubMed: PMC3743898 |
| PLoS One | Recombinant Adiponectin Ameliorates Liver Ischemia Reperfusion Injury via Activating the AMPK/eNOS Pathway PubMed: PMC3676360 |
| The Open Respiratory Medicine Journal | Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias PubMed: PMC3551240 |
| Experimental and Therapeutic Medicine | Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB PubMed: PMC3570159 |
| Journal of Ocular Pharmacology and Therapeutics | Comparison of Acute Effect of Systemic Versus Intravitreal Infliximab Treatment in an Experimental Model of Endotoxin-Induced Uveitis Pubmed: 24116865 |
| Polish Journal of Veterinary Sciences | The effect of different dietary levels of vitamin E and selenium on antioxidant status and immunological markers in serum of laying hens Pubmed: 23971202 |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
| International Immunopharmacology | Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis Pubmed: 24126114 |
| Neurochemical Research | Neonatal morphine administration leads to changes in hippocampal BDNF levels and antioxidant enzyme activity in the adult life of rats Pubmed: 23224818 |
| Journal of natural remedies | Ixora coccinea down Regulate the Inflammation-Associated Prostaglandins (PGE-2), Nitric Oxide (NO) and Cytokines Production in LPS-Stimulated RAW 264.7 Macrophage Jnronline: Source |
| Inflamm Res. | Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats. Pubmed: 24352531 |
| PLoS One. | Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats. Pubmed: 24647044 |
| J Surg Res. | Immunogenic alteration in laparoscopic common bile duct exploration. Pubmed: 24239148 |
| BMC anesthesiology | Repeated open endotracheal suctioning causes gradual desaturation but does not exacerbate lung injury compared to closed endotracheal suctioning in a rabbit model of ARDS. |