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SKU(재고 관리 코드):PAA133Ra01

Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAA133Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA133Ra01-Recombinant Tumor Necrosis Factor Alpha (TNFa)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Experimental Animals Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction Jstage: source
Not available Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia Universi: 49434630
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Respiration Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice Karger: 000319151
Journal of Huazhong University of Science and Technology-Medical Sciences Expression and significance of fgl2 prothrombinase in cardiac microvascular endothelial cells of rats with type 2 diabetes SpringerLink: 161m725t24x85kvv
The European Journal of Clinical Investigation Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes Wiley: source
Journal of Biomedical Research Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy ScienceDirect: S1674830111600158
Stem Cells Combined Effects of Hematopoietic Progenitor Cell Mobilization from Bone Marrow by Granulocyte Colony Stimulating Factor and AMD3100 and Chemotaxis into the Brain Using Stromal Cell-Derived Factor-1α in an Alzheimer's Disease Mouse Model PubMed: 21608078
Journal of Physiology and Biochemistry Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats SpringerLink: h875w73171w2147w
The EMBO Journal Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice Nature: 2011277
Reproductive BioMedicine Online Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages ScienceDirect: S1472648312001484
Journal of Vascular and Interventional Radiology Hepatocellular Necrosis, Apoptosis, and Proliferation after Transcatheter Arterial Embolization or Chemoembolization in a Standardized Rabbit Model PubMed: 21959058
Bone Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration PubMed: 22796416
Journal of Periodontology The Effects of a Novel Botanical Agent on LPS-Induced Alveolar Bone Loss in Rats PubMed: 23106504
Ann Rheum Dis. The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats PubMed: 23172753
Journal of Surgical Research Enhanced tumor growth after portal vein embolization in a rabbit tumor model PubMed: 23149224
Journal of Applied Physiology Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process PubMed: 22984252
Journal of Periodontology Online The Effect of Radachlorin-Mediated Antimicrobial Photodynamic Therapy on Clinical Parameters and Cytokine Profile in Ligature-Induced Periodontitis in Dogs PubMed: 22813345
PLoS One New Method to Isolate and Culture Rat Kupffer Cells PubMed: PMC3743898
PLoS One Recombinant Adiponectin Ameliorates Liver Ischemia Reperfusion Injury via Activating the AMPK/eNOS Pathway PubMed: PMC3676360
The Open Respiratory Medicine Journal Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias PubMed: PMC3551240
Experimental and Therapeutic Medicine Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB PubMed: PMC3570159
Journal of Ocular Pharmacology and Therapeutics Comparison of Acute Effect of Systemic Versus Intravitreal Infliximab Treatment in an Experimental Model of Endotoxin-Induced Uveitis Pubmed: 24116865
Polish Journal of Veterinary Sciences The effect of different dietary levels of vitamin E and selenium on antioxidant status and immunological markers in serum of laying hens Pubmed: 23971202
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
International Immunopharmacology Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis Pubmed: 24126114
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Inflamm Res. Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats. Pubmed: 24352531
PLoS One. Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats. Pubmed: 24647044
J Surg Res. Immunogenic alteration in laparoscopic common bile duct exploration. Pubmed: 24239148
BMC anesthesiology Repeated open endotracheal suctioning causes gradual desaturation but does not exacerbate lung injury compared to closed endotracheal suctioning in a rabbit model of ARDS. Pubmed: 24308643
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