FineTest
SKU(재고 관리 코드):ER1393
Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Product Name
Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Alias
TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.906-250pg/ml
Sensitivity
< 2.344pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-103 |
93 |
|
EDTA plasma(n=5) |
86-104 |
95 |
|
heparin plasma(n=5) |
88-104 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
88-103% |
87-104% |
|
EDTA plasma(n=5) |
87-101% |
84-100% |
82-99% |
|
heparin plasma(n=5) |
87-100% |
90-93% |
82-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
