This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using TOP2A (Phospho-Ser1106) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from Jurkat cells treated with paclitaxel 1uM 24h, using TOP2A (Phospho-Ser1106) Antibody. The lane on the right is blocked with the phospho peptide.