YWHAG; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; KCIP-1
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human 14-3-3 gamma. AA range:51-100
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
28kD
GeneID（Human）:
7532
Human Swiss-Prot No:
P61981
Cellular localization:
Cytoplasm .
Background:
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008],
Immunofluorescence analysis of COS7 cells, using 14-3-3 gamma Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 14-3-3 gamma Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from K562 cells, treated with insulin 0.01U/ml 15', using 14-3-3 gamma Antibody. The lane on the right is blocked with the synthesized peptide.