53BP1 Polyclonal Antibody

The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.

Introducing the 53BP1 Polyclonal Antibody, a highly reliable and efficient tool designed for advanced research applications. This meticulously developed antibody is specifically tailored to target the 53BP1 protein, a crucial component involved in DNA damage response and repair mechanisms.

Crafted with utmost precision, this polyclonal antibody exhibits exceptional specificity and sensitivity, enabling accurate detection and analysis of 53BP1 protein expression levels in various biological samples. Its superior affinity ensures optimal binding capacity, facilitating robust and reproducible results.

The 53BP1 Polyclonal Antibody boasts a wide range of applications, including immunohistochemistry (IHC), immunofluorescence (IF), western blotting (WB), and flow cytometry (FC). Its versatility allows for comprehensive investigations into the intricate molecular pathways associated with DNA damage response, cell cycle regulation, and tumorigenesis.

With its user-friendly formulation, this antibody guarantees ease of use and seamless integration into diverse experimental protocols. Its exceptional stability and long shelf life ensure consistent performance, making it an indispensable asset for laboratories and research institutions alike.

Choose the 53BP1 Polyclonal Antibody to unlock the full potential of your research endeavors. Experience unparalleled accuracy, reliability, and efficiency in unraveling the complexities of DNA damage response mechanisms. Elevate your scientific pursuits with this cutting-edge tool, revolutionizing the field of molecular biology.