Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Acrp30. AA range:6-55
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
30kD
GeneID（Human）:
9370
Human Swiss-Prot No:
Q15848
Cellular localization:
Secreted .
Background:
adiponectin, C1Q and collagen domain containing(ADIPOQ) Homo sapiens This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010],
Western blot analysis of lysates from 1) HeLa, 2) VEC, 3) 293 cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） Tubulin β Monoclonal Antibody(5G3) (cat:YM3030) anti
Western Blot analysis of various cells using Acrp30 Polyclonal Antibody diluted at 1:1000
Western Blot analysis of HeLa cells using Acrp30 Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysate from HeLa cells, using Acrp30 antibody.