Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
Synthesized peptide derived from Actin-α cardiac muscle . at AA range: 1-80
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
42kD
GeneID（Human）:
70
Human Swiss-Prot No:
P68032
Cellular localization:
Cytoplasm, cytoskeleton.
Background:
Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008],
Western Blot analysis of mouse heart, mouse lung cells using Actin-α cardiac muscle Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100