Cloud-clone
SKU(재고 관리 코드):APA134Hu01
Active Tumor Necrosis Factor Beta (TNFb)
Active Tumor Necrosis Factor Beta (TNFb)
Product No.
APA134Hu01
Organism Species
Homo sapiens (Human).
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
9.3
Applications
Cell culture; Activity Assays.
UOM
10µg 50µg 200µg 1mg 5mg
ACTIVITY TEST
TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-β could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10ng/mL) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA .
Cell necrosis after incubation with TNF-β(10ng/mL) for 72h was shown in Figure 1.
The production of IL-1β after incubation with TNF-β (10ng/mL) for 8h is shown in Table 1.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process PubMed: 22984252 |
| Journal of Translational Medicine | Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation Pubmed:26847569 |
| International Journal of Stem Cell Research and Transplantation | Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines IJST-2328-3548-04-803 |
| Probiotics Antimicrob Proteins | Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … Pubmed:29305686 |
| Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … Pubmed: 32680553 |
| Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort | |
| BMC MUSCULOSKELETAL DISORDERS | Elevated lymphotoxin-¦Á (TNF¦Â) is associated with intervertebral disc degeneration 33441130 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA134Hu01 | Active Tumor Necrosis Factor Beta (TNFb) | Cell culture; Activity Assays. |
| RPA134Hu01 | Recombinant Tumor Necrosis Factor Beta (TNFb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA134Hu01 | Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb) | WB; IHC; ICC; IP. |
| MAA134Hu22 | Monoclonal Antibody to Tumor Necrosis Factor Beta (TNFb) | WB; IHC; ICC; IP. |
| MAA134Hu21 | Monoclonal Antibody to Tumor Necrosis Factor Beta (TNFb) | WB; IHC; ICC; IP. |
| SEA134Hu | ELISA Kit for Tumor Necrosis Factor Beta (TNFb) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA134Hu | Magnetic Luminex Assay Kit for Tumor Necrosis Factor Beta (TNFb) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
