anti- Aconitase 1 antibody

Illuminate the roles of Aconitase 1 in the citric acid cycle with our Anti-Aconitase 1 Antibody. With a substantial 100µg supply, this antibody empowers you to delve into Aconitase 1 and its pivotal role in cellular energy metabolism.

Aconitase 1, a critical enzyme in the citric acid cycle, plays a pivotal role in converting citrate to isocitrate, a fundamental step in energy production. Our antibody is precision-engineered to specifically target Aconitase 1 with remarkable precision and sensitivity. Thoroughly validated, it ensures consistent and dependable results, instilling confidence in your exploration of cellular energy production.

Utilizing state-of-the-art techniques, this antibody is sourced from rabbit hosts, offering a polyclonal clonality that broadens its range of binding capabilities. Its versatility makes it an ideal choice for various applications, including Western blotting, immunohistochemistry, and immunofluorescence assays.

Whether you're unraveling the citric acid cycle mechanisms, studying mitochondrial metabolism, or exploring diseases related to Aconitase 1 dysregulation, our Anti-Aconitase 1 Antibody is your steadfast research companion. Its high-quality formulation and generous 100µg size ensure it is an indispensable asset for your laboratory, enabling groundbreaking discoveries and contributions to cellular metabolism research.

Empower your research with the precision and reliability of our Anti-Aconitase 1 Antibody. Secure your 100µg supply today and embark on a journey to uncover the intricacies of energy metabolism.

Product Name

Aconitase 1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants

IMMUNOGEN INFORMATION

Immunogen

aconitase 1, soluble

Synonyms

ACO1, Aconitase, Aconitase 1, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1

Observed MW

120 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:100

UNIPROT INFORMATION

UniProt ID

P21399

IMAGES

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using FNab00087(ACO1 antibody) at dilution of 1:100

Hela cells were subjected to SDS PAGE followed by western blot with FNab00087(ACO1 antibody) at dilution of 1:1000