anti- AFF4 antibody

Presenting our advanced Anti-AFF4 Antibody, available in a convenient 100µg size, meticulously crafted to drive your research in the dynamic field of transcriptional regulation and gene expression.

The Anti-AFF4 Antibody is designed to meet the stringent demands of the scientific community. AFF4 (AF4/FMR2 Family Member 4) is a key player in transcriptional elongation and the formation of the super elongation complex (SEC), making it a central focus in studies of gene regulation. Our antibody offers exceptional specificity and sensitivity, rendering it an ideal choice for various applications, from chromatin immunoprecipitation to RNA polymerase II activity assays and more.

What truly distinguishes our Anti-AFF4 Antibody is its exceptional performance. It has undergone rigorous testing and validation to ensure consistent and reproducible results, effectively reducing experimental variability and saving you valuable time and resources.

With a 100µg size, this product strikes the perfect balance between cost-effectiveness and usability. You'll have an ample supply of antibody for multiple experiments while minimizing wastage, aligning with our commitment to sustainable research practices.

Engineered for user-friendliness, our Anti-AFF4 Antibody is suitable for researchers at all levels, from newcomers to seasoned experts. It seamlessly integrates into various laboratory techniques, enhancing the precision and reliability of your experiments.

Empower your research with confidence, knowing you have the support of a high-quality, meticulously crafted Anti-AFF4 Antibody at your fingertips. Step into the forefront of scientific discovery in transcriptional regulation and gene expression with a dependable partner by your side. Trust in our unwavering dedication to quality and innovation as you unravel the complexities of AFF4's roles in controlling gene expression and cellular function.

Product Name

AFF4 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Key component of the super elongation complex(SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins(ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.

IMMUNOGEN INFORMATION

Immunogen

AF4/FMR2 family, member 4

Synonyms

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31

Observed MW

150 kDa

APPLICATION

Tested Application

ELISA, WB, IP

Recommended Dilution

WB: 1:200-1:2000; IP: 1:200-1:1000

UNIPROT INFORMATION

UniProt ID

Q9UHB7

IMAGES

IP Result of anti-AFF4 (IP:FNab00197, 4ug; Detection:FNab00197 1:300) with HepG2 cells lysate 3200ug.

L02 cells were subjected to SDS PAGE followed by western blot with FNab00197(AFF4 antibody) at dilution of 1:500