anti- Caspase 4 antibody

Our Anti-Caspase 4 Antibody (100µg) is a valuable tool for researchers studying apoptosis and inflammation. Caspase 4, also known as Caspase 11 in mice, is a critical player in the innate immune response and pyroptosis. This antibody, targeting Caspase 4, ensures precise and reliable results in your experiments.

With 100µg of this antibody per vial, you'll have a sufficient supply for multiple experiments, reducing the need for frequent reordering. Whether you're conducting Western blots, immunohistochemistry, or flow cytometry, this antibody consistently delivers high-quality performance.

Researchers can trust the specificity and sensitivity of this antibody, which is produced using advanced techniques and high-quality materials. Its reliability makes it an indispensable asset for projects aiming to elucidate the role of Caspase 4 in immune responses and cell death pathways.

Advance your research on apoptosis and inflammation with confidence using our Anti-Caspase 4 Antibody. By utilizing this antibody, you contribute to the progress of scientific knowledge in these crucial fields.

Product Name

Caspase 4 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Inflammatory caspase, essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS(PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators(PubMed:24879791, PubMed:25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection(PubMed:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity(PubMed:26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains(PubMed:15123740, PubMed:22246630, PubMed:23661706). Activated by direct binding to LPS without the need of an upstream sensor(PubMed:25119034). Does not directly process IL1B(PubMed:7743998, PubMed:7797592, PubMed:7797510).

IMMUNOGEN INFORMATION

Immunogen

caspase 4, apoptosis-related cysteine peptidase

Synonyms

CASP 4, CASP4, Caspase 4, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX

Observed MW

43 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P49662

IMAGES