anti- CHCHD4 antibody

Our Anti-CHCHD4 Antibody is a powerful tool for researchers seeking to unravel the mysteries of mitochondrial biology. This 100µg vial contains a highly specific polyclonal antibody designed to target the CHCHD4 protein, a crucial player in mitochondrial function regulation.

CHCHD4, also known as Mia40, plays a pivotal role in the import and folding of proteins within the mitochondria, the powerhouse of the cell. Dysregulation of CHCHD4 has been linked to various mitochondrial disorders. Our antibody is rigorously quality-tested to ensure reliability and specificity in your experiments.

With a dedication to scientific excellence, we provide researchers with a dependable tool to advance their studies in mitochondrial biology, biochemistry, and cell biology. Trust in our Anti-CHCHD4 Antibody to deliver precise results, facilitating your groundbreaking discoveries.

Product Name

CHCHD4 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17 or MICU1(PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364). Required for the import and folding of small cysteine-containing proteins(small Tim) in the mitochondrial intermembrane space(IMS)(PubMed:16185709, PubMed:19182799, PubMed:21059946). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria(PubMed:16185709, PubMed:19182799, PubMed:21059946). The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS(PubMed:16185709, PubMed:19182799, PubMed:21059946). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system(PubMed:23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake(PubMed:26387864).

IMMUNOGEN INFORMATION

Immunogen

coiled-coil-helix-coiled-coil-helix domain containing 4

Synonyms

MIA40

Observed MW

22-25 kDa

APPLICATION

Tested Application

ELISA, IHC, IF, WB, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q8N4Q1

IMAGES