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    BPS Bioscience

    101676-2

    Anti-Claudin-18 Isoform 2 Antibody, PE-Labeled 100 ug

    Anti-Claudin-18 Isoform 2 Antibody, PE-Labeled 100 ug

    ●Description

    This anti-Claudin-18 isoform 2 (CLDN18.2) antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested in flow cytometry assay for specific binding to human CLDN18.2 on the surface of CLDN18.2 CHO Recombinant Cell Line (BPS Bioscience #78533).

    ●Synonyms
    Claudin-18, CLDN18, UNQ778, PRO1572
    ●Product Data Gallery

    Product Info
    Species : Human

    Isotype : IgG1

    Clonality : Monoclonal

    Host Species/Expression System : HEK293

    Purity : ≥90%

    Purification : Protein A affinity chromatography from HEK293 supernatants.

    Format : Aqueous buffer solution.

    Formulation : 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain protein stabilizer.

    Concentration : The concentration will vary with each lot; the exact value will be provided with each shipment.

    UniProt # : P56856

    Label : PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa).

    Storage/Stability : At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C. 

    Assay Conditions : One million CLDN18.2 CHO recombinant cells (BPS Bioscience #78533) were blocked and stained with 1 µg of anti-CLDN18.2 antibody, PE-labeled (BPS Bioscience #101676) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CLDN18.2 CHO recombinant cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.

    Applications : Useful for studying expression of CLDN18.2 on the cell surface in flow cytometry assay. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

    Shipping Temperature : +4°C (gel packs)

    Cross Reactivity : This antibody recognizes human CLDN 18.2. It has not been tested with other species.

    Warnings : Do not freeze. Protect from light.

    Scientific Category : Immunotherapy

    Regulatory Status : Research Use Only
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