Kerafast
SKU(재고 관리 코드):EWS101
Anti-CREB-H (CREB3L3) Antibody
Anti-CREB-H (CREB3L3) Antibody
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This rabbit IgG polyclonal antibody was generated against purified protein segment, N-terminal amino acids 75 to 250 of mouse cAMP-responsive element binding protein, hepatocyte specific (CREB-H) protein and reacts against human and mouse CREB-H.
Highlights
- Exhibits high specificity and high titer
- Reacts with human and mouse CREBH
- Suitable for Western Blot, Immunoprecipitation and ChIP analysis applications
CREB-H (also called CREB3L3) is a stress sensor that plays key roles in lipid and glucose metabolism associated with metabolic disease. CREB-H is a liver-specific bZIP transcription factor with molecular weights of 52.7 kD (precursor predicted size) and 35.3 kD (activated form predicted size). The CREB-H protein is localized to the endoplasmic reticulum and acts as a transcription factor activated by cyclic AMP stimulation.
From the laboratory of Kezhong Zhang, PhD, Wayne State University.
| Product Type: | Antibody |
| Antigen: | CREB-H, CREB3L3 |
| Accession ID: | Q68CJ9 |
| Molecular Weight: | 52.7 kDa (predicted) |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Human and mouse |
| Immunogen: | Purified protein segment, N-terminal 176 amino acids |
| Species Immunized: | Rabbit |
| Epitope: | AA 75 - 250 of CREB-H protein |
| Purification Method: | Affinity Purified |
| Buffer: | PBS/0.05% Azide, 50% Glycerol |
| Tested Applications: | WB (1:1000-2000), IP-Western (1:1000), ChIP (1:1000) |
| Storage: | -20C |
| Shipped: | Cold packs |
Notes
For Western blot analysis with total raw lysates from liver tissues, the antibody can detect an unspecific signal around 90 kD when films are incubated with PDVF membranes for a relatively long time. But the unspecific signal can be easily separated or distinguished from the specific signals of CREB-H precursor (around 75 kD gel size) and activated (around 50 kD gel size) forms.
IP-Western Blot Analysis
IP-Western blot analysis of CREB-H and PPAR? interaction in the livers of mice under normal chow, after 14-hour fasting, or after 6 months of AHF diet. Whole-protein lysates were prepared from mouse liver tissues and subjected to IP to pull down the endogenous PPAR? or CREB-H protein complex using an anti-PPAR? or anti-CREB-H antibody. The precipitates were analyzed by immunoblotting with the antibody against CREB-H or PPAR?. Whole-liver protein lysates were subjected to Western blot analysis to determine the levels of CREB-H, PPAR?, and ?-actin as the controls. The values below the gels represent the interactive CREB-H (with PPAR?) and PPAR? (with CREB-H) protein signal intensities after normalization to ?-actin signal intensities. Ab, antibody; Ad, adenovirus.
Adapted from: Kim, H., et al. Endocrinology 155: 769-782. 2014 Mar;155(3):769-82.
- Kim, H., Mendez, R., Zheng, Z., Chang, L., Cai, J., Zhang, R., and Zhang, K. 2014. Liver-enriched Transcription Factor CREBH Interacts with Peroxisome Proliferator-activated Receptor ? to Regulate Metabolic Hormone FGF21. Endocrinology 155: 769-782. 2014 Mar;155(3):769-82
- Zhang, C., Wang, G., Zheng, Z., Maddipati, K.R., Zhang, X., Dyson, G., Williams, P., Duncan, S.A., Kaufman, R.J., and Zhang, K. 2012. ER-tethered Transcription Factor CREBH Regulates Hepatic Lipogenesis, Fatty Acid Oxidation, and Lipolysis upon Metabolic Stress. Hepatology 55 (4): 1070-1082. PMCID: PMC3319338.