anti- DDB2 antibody

Our Anti-DDB2 (DNA Damage-Binding Protein 2) Antibody, available in a 100µg size, is a valuable tool for researchers exploring the role of DDB2 in DNA repair mechanisms and its implications in genome stability and cancer development. DDB2 is a key component of the DDB1-CUL4A-RBX1 E3 ubiquitin ligase complex, which plays a pivotal role in nucleotide excision repair.

Accurate detection of DDB2 is essential for understanding its functions and its potential involvement in DNA damage response pathways. This antibody has undergone rigorous validation for specificity and sensitivity, ensuring reliable results in applications such as chromatin immunoprecipitation and immunohistochemistry.

With its 100µg size, this antibody provides ample material for multiple experiments, enabling you to delve deeper into DDB2's expression and function in different cell types and under various genotoxic stress conditions. Trust in the quality of our Anti-DDB2 Antibody to advance your research and gain insights into the complex mechanisms of DNA repair and genome maintenance.

Product Name

DDB2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex(the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway(the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers(CPD), 6-4 photoproducts(6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX(DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1(also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.

IMMUNOGEN INFORMATION

Immunogen

damage-specific DNA binding protein 2, 48kDa

Observed MW

55-60 kDa

APPLICATION

Tested Application

ELISA, WB, IF

Recommended Dilution

WB: 1:500-1:2000; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q92466

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