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SKU(재고 관리 코드):FNab02549

anti- DTL antibody

anti- DTL antibody

size

Uncover the mysteries of cellular biology with our meticulously crafted Anti-DTL Antibody, now available in a 100µg size. Dihydrolipoamide S-succinyltransferase (DTL) is a multifunctional enzyme involved in critical metabolic pathways, making it a compelling target for researchers studying mitochondrial function, energy metabolism, and cellular respiration.

This highly specific antibody empowers you to delve into the intricacies of DTL and its roles with precision and confidence. It has undergone rigorous quality control to ensure accuracy and reproducibility in your experiments, making it the ideal choice for researchers investigating metabolic diseases, mitochondrial biology, and energy production.

DTL is a key player in the tricarboxylic acid cycle and is associated with various metabolic disorders and energy production processes. Whether you're researching metabolic diseases, mitochondrial function, or cellular respiration, this antibody will be your trusted companion.

Product Name

DTL antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Substrate-specific adapter of a DCX(DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBXO18/FBH1 and KMT5A(PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication(PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing(PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration(PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis(PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613).

IMMUNOGEN INFORMATION

Immunogen

denticleless homolog(Drosophila)

Synonyms

CDT2, CDW1, DCAF2, L2DTL, RAMP

Observed MW

79 kDa

APPLICATION

Tested Application

ELISA, WB, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:2000

UNIPROT INFORMATION

UniProt ID

Q9NZJ0

IMAGES

 

IP Result of anti-DTL (IP:FNab02549, 3ug; Detection:FNab02549 1:500) with mouse testis tissue lysate 8000ug.
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02549(DTL antibody) at dilution of 1:200

 

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