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FineTest

FNab02847

anti- ERK1/2 antibody

anti- ERK1/2 antibody

size

Unleash the power of research with our Anti-ERK1/2 Antibody, an essential tool for investigating the intricate signaling pathways regulated by the extracellular signal-regulated kinases 1 and 2 (ERK1/2). With 100µg of high-quality antibody, you have the precision and reliability required for your experiments.

ERK1 and ERK2 are central players in the mitogen-activated protein kinase (MAPK) pathway, regulating various cellular processes such as proliferation, differentiation, and survival. Our antibody is rigorously tested for specificity and sensitivity, making it ideal for techniques such as immunoblotting, immunoprecipitation, and immunofluorescence.

The Anti-ERK1/2 Antibody is your indispensable tool for unraveling the roles of ERK1/2 in cell signaling and their contributions to numerous physiological and pathological conditions. Whether you're involved in basic research or investigating diseases related to MAPK signaling, this antibody is an invaluable asset, providing you with the confidence to advance your scientific inquiries and make significant strides in your work.

Product Name

ERK1/2 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF.

IMMUNOGEN INFORMATION

Immunogen

mitogen-activated protein kinase 3

Synonyms

ERK, ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

Observed MW

38-44 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB：1:500-1:5000; IP: 1:1000-1:10000; IHC: 1:100-1:400; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P27361

IMAGES

 

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using FNab02847(ERK1/2 Antibody) at dilution of 1:200. Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
Immunofluorescent analysis of HepG2 cells using FNab02847(ERK1/2 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02847(ERK1/2 Antibody) at dilution of 1:1000

 

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