anti- GAPDH antibody

Uncover the secrets of cellular metabolism with our Anti-GAPDH Antibody. This vital research tool empowers you to explore the intricacies of GAPDH-related studies. Key features include:

• High Purity: Each 100µg vial contains highly purified antibodies, ensuring precise and reproducible results.
• Specificity: This antibody is designed to specifically target Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), making it ideal for various research applications.
• Versatile Usage: Suitable for techniques such as Western blotting, immunohistochemistry, and immunoprecipitation, this antibody accommodates diverse research needs.
• Metabolic Insights: Gain valuable insights into glycolysis, energy production, and the significance of GAPDH in various cellular processes.

Elevate your cell biology research with our Anti-GAPDH Antibody, your key to understanding the world of cellular metabolism.

Product Name

GAPDH antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation.

IMMUNOGEN INFORMATION

Immunogen

glyceraldehyde-3-phosphate dehydrogenase

Synonyms

GAPD,G3PD,GAPDH, OK/SW cl.12

Observed MW

36 kDa

APPLICATION

Tested Application

ELISA, IHC, WB

Recommended Dilution

WB: 1:100-1:5000; IHC: 1:100-1:500

UNIPROT INFORMATION

UniProt ID

P04406

IMAGES