anti- GAPDH antibody

Delve into the world of cellular metabolism and energy production with our Anti-GAPDH Antibody. This indispensable research tool empowers you to investigate GAPDH-related studies. Key features include:

• High Specificity: Each 100µg vial contains antibodies meticulously designed to specifically target Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), ensuring accuracy and reliability in your experiments.
• Enhanced Sensitivity: This antibody is highly sensitive, allowing for the precise detection of GAPDH in various experimental setups.
• Versatile Applications: Suitable for Western blotting, immunocytochemistry, and immunoprecipitation, this antibody accommodates diverse research methodologies.
• Metabolic Insights: Gain valuable insights into GAPDH's role in glycolysis, its significance in cellular metabolism, and its implications in various diseases and cellular processes.

Empower your studies in cell biology and biochemistry with our Anti-GAPDH Antibody, your key to unraveling the complexities of cellular metabolism and energy production.

With our high-quality antibodies, you'll have the tools you need to advance your research, gain valuable insights, and make meaningful contributions to your field.

Product Name

GAPDH antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues.

IMMUNOGEN INFORMATION

Immunogen

glyceraldehyde-3-phosphate dehydrogenase

Synonyms

G3PD, GAPD, GAPDH

Observed MW

36 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IF, FC, IP

Recommended Dilution

WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P04406

IMAGES