anti- GFP tag antibody

Explore the world of molecular biology and protein expression with our Anti-GFP Tag Polyclonal Antibody. This versatile antibody is designed for detecting and studying proteins fused with the Green Fluorescent Protein (GFP) tag, providing researchers with a valuable tool for various applications. Key features include:

• Polyclonal Advantage: The use of a polyclonal antibody offers a higher likelihood of recognizing multiple epitopes on the GFP tag, enhancing its sensitivity and versatility.

• Broad Applications: Suitable for a wide range of research methodologies, including Western blotting, immunoprecipitation, and immunofluorescence, this antibody is a valuable addition to your molecular biology toolkit.

• Precise GFP Tag Recognition: This antibody is meticulously engineered to specifically target the GFP tag, ensuring accurate and reliable results in your experiments.

• 100µg Size: The 100µg size provides ample supply for multiple experiments, ensuring that you have the reagents you need to drive your research forward.

Empower your research with our Anti-GFP Tag Polyclonal Antibody, offering the ability to detect and analyze GFP-tagged proteins with confidence and versatility.

Product Name

GFP tag antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

IMMUNOGEN INFORMATION

Immunogen

GFP tag

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, YFP

Observed MW

30 kDa

APPLICATION

Tested Application

ELISA, WB, IP

Recommended Dilution

WB: 1:1000-1:4000; IP: 1:2000-1:20000

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