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MBL Life science

M180-11

Anti-HA-tag mAb-Magnetic Beads

Anti-HA-tag mAb-Magnetic Beads

Data

  • Immunoprecipitation
Clonality Monoclonal Clone TANA2
Isotype (Immunized Animal) Mouse IgG2bκ
Applications
IP       50 µL/sample  
RIP*    reported.  （PMID: 28959057）
Immunogen (Antigen) KLH conjugated synthetic peptide, YPYDVPDYA (HA-tag)
Storage buffer PBS/0.1% BSA/0.09% NaN3
Storage temp. 4°C Conjugate Magnetic Beads Manufacturer MBL
Alternative names Human influenza hemagglutinin
Background

Epitope tag technique has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation, and immunofluorescence, utilizing with an antibody that recognizes such an epitope. HA-tag (YPYDVPDYA) sequence is widely used for the epitope tag, which corresponding to the partial peptide of Influenza hemagglutinin protein.
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Citations

Immunoprecipitation

  1. Shichino Y et al. YTH-RNA-binding protein prevents deleterious expression of meiotic proteins by tethering their mRNAs to nuclear foci. Elife 7, e32155 (2018)(PMID:29424342)
  2. Kato K et al. Pulmonary pericytes regulate lung morphogenesis. Nat Commun. 9, 2448 (2018)(PMID:29934496)

RNP Immunoprecipitation

  1. Jeong HW et al. Transcriptional regulation of endothelial cell behavior during sprouting angiogenesis. Nat Commun. 8, 726 (2017)(PMID:28959057)
  2. Bae H et al. Angiopoietin-2-integrin α5β1 signaling enhances vascular fatty acid transport and prevents ectopic lipid-induced insulin resistance. Nat Commun. 11, 2980 (2020)(PMID:32532986)
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