anti- LDLR antibody

anti- LDLR antibody

Product Name

LDLR antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

IMMUNOGEN INFORMATION

Immunogen

low density lipoprotein receptor

Synonyms

FH, FHC, LDL receptor, LDLR

Observed MW

150-160 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P01130

IMAGES

Immunohistochemistry of paraffin-embedded human brain tissue slide using FNab04742(LDLR Antibody) at dilution of 1:200 heat mediated antigen retrieved with Sodium Citrate buffer(pH6).

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab04742 (LDLR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab04742(LDLR antibody) at dilution of 1:300