anti- MCC antibody

anti- MCC antibody

Product Name

MCC antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase(PAK) activation(PubMed:18591935, PubMed:19555689, PubMed:22480440). Represses the beta-catenin pathway(canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin(CTNB1) transcriptional activity(PubMed:24824780).

IMMUNOGEN INFORMATION

Immunogen

mutated in colorectal cancers

Observed MW

98 kDa ; 113-118 kDa

APPLICATION

Tested Application

ELISA, IHC, IP, WB, IF

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P23508

IMAGES

Immunohistochemistry of paraffin-embedded human prostate cancer using FNab05046(MCC antibody) at dilution of 1:100

IP Result of anti-MCC (IP:FNab05046, 3ug; Detection:FNab05046 1:500) with PC-3 cells lysate 5000ug.

human brain tissue were subjected to SDS PAGE followed by western blot with FNab05046(MCC antibody) at dilution of 1:500