anti- MCM7 antibody

anti- MCM7 antibody

Product Name

Product Name

MCM7 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Acts as component of the MCM2-7 complex(MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

IMMUNOGEN INFORMATION

Immunogen

minichromosome maintenance complex component 7

Synonyms

CDC47, MCM2

Observed MW

81 kDa

APPLICATION

Tested Application

ELISA, WB, IP, IHC

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P33993

IMAGES

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab05062(MCM7 Antibody) at dilution of 1:50

IP Result of anti-MCM7 (IP:FNab05062, 4ug; Detection:FNab05062 1:2000) with NIH/3T3 cells lysate 1600ug.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab05062(MCM7 Antibody) at dilution of 1:3000