anti- MECP2 antibody

anti- MECP2 antibody

Product Name

MECP2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms.

IMMUNOGEN INFORMATION

Immunogen

methyl CpG binding protein 2 (Rett syndrome)

Synonyms

AUTSX3

Observed MW

83 kDa

APPLICATION

Tested Application

ELISA, IHC, WB

Recommended Dilution

WB: 1:500 - 1:1000; IHC: 1:50 - 1:100

UNIPROT INFORMATION

UniProt ID

P51608

Immunohistochemistry of paraffin-embedded human gastric cancer using FNab05082(MECP2 antibody) at dilution of 1:50

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with FNab05082(MECP2 Antibody) at dilution of 1:1000