anti- MSH6 antibody

anti- MSH6 antibody

Product Name

MSH6 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3(H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

IMMUNOGEN INFORMATION

Immunogen

mutS homolog 6

Synonyms

G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160

Observed MW

160 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P52701

IMAGES

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab05376(MSH6 Antibody) at dilution of 1:100

IP Result of anti-MSH6 (IP:FNab05376, 4ug; Detection:FNab05376 1:600) with HEK-293 cells lysate 1800ug.

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab05376(MSH6 Antibody) at dilution of 1:1000

RELATED PRODUCTS

• MSH6 antibody
• MSH6 antibody

STATEMENT

Manuals on the web are for your reference only, specifications are subject to the delivery manuals.

FEATURED PRODUCT