anti- NANOG antibody

anti- NANOG antibody

Product Name

NANOG antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Transcription regulator involved in inner cell mass and embryonic stem(ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating(ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

IMMUNOGEN INFORMATION

Immunogen

Nanog homeobox

Synonyms

hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox

Observed MW

39-48 kDa

APPLICATION

Tested Application

ELISA, IF, WB

Recommended Dilution

WB: 1:500-1:2000; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9H9S0

IMAGES

Mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05541( NANOG Antibody) at dilution of 1:1000