anti- NENF antibody

PRODUCT INFORMATION

Product Name

NENF antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance(By similarity). Plays a role in the human tumorigenesis(PubMed:22748190).

IMMUNOGEN INFORMATION

Immunogen

neuron derived neurotrophic factor

Synonyms

CIR2, NENF, NEUDESIN, SCIRP10, SPUF, SPUF protein

Observed MW

18 kDa

APPLICATION

Tested Application

ELISA, IHC, WB,IF

Recommended Dilution

WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

Q9UMX5

IMAGES

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using FNab05661 ( NENF Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

human brain tissue were subjected to SDS PAGE followed by western blot with FNab05661(NENF antibody) at dilution of 1:1000