anti- P4HA1 antibody

PRODUCT INFORMATION

Product Name

P4HA1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Catalyzes the post-translational formation of 4-hydroxyproline in-Xaa-Pro-Gly-sequences in collagens and other proteins.

IMMUNOGEN INFORMATION

Immunogen

prolyl 4-hydroxylase, alpha polypeptide I

Synonyms

4 PH alpha 1, P4HA, P4HA1

Observed MW

61 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP, IF

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P13674

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed A431 cells using FNab06078 (P4HA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

IP Result of anti-P4HA1 (IP:FNab06078, 3ug; Detection:FNab06078 1:600) with A431 cells lysate 3300ug.

A431 cells were subjected to SDS PAGE followed by western blot with FNab06078(P4HA1 antibody) at dilution of 1:600