anti- Phospho-Akt(Ser473) antibody

PRODUCT INFORMATION

Product Name

Phospho-Akt(Ser473) antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification.

IMMUNOGEN INFORMATION

Immunogen

v-akt murine thymoma viral oncogene homolog 1

Synonyms

AKT, AKT1, AKT1-phospho-S473, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

Observed MW

60KD

APPLICATION

Tested Application

ELISA, IHC, WB

Recommended Dilution

WB: 1:1000-1:4000; IHC: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P31749

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab06402(Phospho-Akt (S473) Antibody) at dilution of 1:2000