anti- PKM1-specific antibody

PRODUCT INFORMATION

Product Name

PKM1-specific antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate(PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2(PMID:18337823). This antibody is specific to PKM1 isoform.

IMMUNOGEN INFORMATION

Immunogen

PKM2 pyruvate kinase, muscle

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

Observed MW

58KD

APPLICATION

Tested Application

ELISA, WB, IHC, IP, IF

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:100-1:400; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P14618

Immunofluorescent analysis of HeLa cells using FNab06494 (PKM1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

IP Result of anti-PKM1-specific (IP:FNab06494, 3ug; Detection:FNab06494 1:1000) with mouse brain tissue lysate 4000ug.

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab06494(PKM1 antibody) at dilution of 1:1000