anti- SHARPIN antibody

Unleash the power of ubiquitin-mediated signaling and cellular homeostasis research with our Anti-SHARPIN Antibody. Available in a 100µg size, this antibody is your essential tool for understanding the role of SHANK-associated RH domain-interacting protein (SHARPIN) in protein turnover and immune responses. Key Features: Precise Targeting: This antibody is rigorously validated for specific recognition of SHARPIN, ensuring accurate and reliable results in your studies of protein degradation, ubiquitination, and immune regulation. Versatile Applications: Compatible with a variety of research techniques, including Western blotting, immunoprecipitation, and immunohistochemistry. It's ideal for researchers studying immune responses, inflammatory diseases, and proteostasis. High Sensitivity: The Anti-SHARPIN Antibody is optimized for superior sensitivity, enabling you to detect low-abundance SHARPIN in complex biological samples, facilitating a deeper understanding of cellular homeostasis. Broad Research Impact: From unraveling immune regulation to exploring protein quality control mechanisms, this antibody is a valuable asset for researchers in immunology, cell biology, and disease mechanisms. Incorporate the Anti-SHARPIN Antibody into your research to gain deeper insights into the role of SHARPIN in immune responses, inflammation, and cellular homeostasis. Its precision and reliability make it an indispensable asset for scientists and researchers across diverse disciplines.

Product Name

SHARPIN antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex(TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.

IMMUNOGEN INFORMATION

Immunogen

SHANK-associated RH domain interactor

Synonyms

hSIPL1, SHARPIN, SIPL1

Observed MW

40 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

Q9H0F6

IMAGES