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AntibodySystem

SKU(재고 관리 코드):KDA30301

Denosumab ELISA Kit

Denosumab ELISA Kit

Title

Catalog No.: KDA30301

Catalog No. KDA30301
Product name Denosumab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human CD254 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Denosumab in the sample competitively binds to the pre-coated protein with biotin-labeled Denosumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Denosumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Denosumab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 12.21 ng/mL
Range 23.44 - 1,500 ng/mL
Recovery 80-120%
Alternative Names AMG162, HL2001,CAS: 615258-40-7
Shipping 2-8 ℃
Note For Research Use Only.
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