• Nesvacumab ELISA Kit
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AntibodySystem

KDA42302

Nesvacumab ELISA Kit

Nesvacumab ELISA Kit

Title

Catalog No.: KDA42302

Catalog No. KDA42302
Product name Nesvacumab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human ANGPT2 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Nesvacumab in the sample competitively binds to the pre-coated protein with biotin-labeled Nesvacumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Nesvacumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Nesvacumab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 19.13 ng/mL
Range 31.25 - 2,000 ng/mL
Recovery 80-120%
Background Nesvacumab (REGN910) is a fully human immunoglobulin G1 (IgG1) monoclonal antibody that specifically binds and inactivates the Tie2 receptor ligand Ang2 with high affinity, but shows no binding to Ang1. The main objectives of this trial were to determine the safety, tolerability, dose-limiting toxicities (DLT), and recommended phase II dose (RP2D) of nesvacumab.
Alternative Names REGN-910, CAS: 1296818-77-3
Shipping 2-8 ℃
Note For Research Use Only.
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