• Infliximab ELISA Kit
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AntibodySystem

KDB94404

Infliximab ELISA Kit

Infliximab ELISA Kit

Title

Catalog No.: KDB94404

Catalog No. KDB94404
Product name Infliximab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human TNFa has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Infliximab in the sample competitively binds to the pre-coated protein with biotin-labeled Infliximab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Infliximab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Infliximab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 71.38 ng/mL
Range 93.75 - 3,000 ng/mL
Recovery 80-120%
Background Infliximab is a chimeric monoclonal antibody and used to treat auto- immune disorders. Infliximab reduces the amount of active human tumour necrosis factor alpha (hTNFa) in the body by binding to it and preventing it from signaling the receptors for TNFa on the surface of various cell types. TNFa is one of the key cytokines that triggers and sustains the inflammatory reactions. Infliximab is used for the treatment of psoriasis, Crohn’s disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis, ulcerative colitis.
Alternative Names TA-650, cA2, CAS: 170277-31-3
Shipping 2-8 ℃
Note For Research Use Only.
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