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AntibodySystem

SKU(재고 관리 코드):KDB94405

Etanercept ELISA Kit

Etanercept ELISA Kit

Title

Catalog No.: KDB94405

Catalog No. KDB94405
Product name Etanercept ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human TNFa has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Etanercept in the sample competitively binds to the pre-coated protein with biotin-labeled Etanercept. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Etanercept bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Etanercept concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 30.07 ng/mL
Range 31.25 - 2,000 ng/mL
Recovery 80-120%
Alternative Names CAS: 185243-69-0
Shipping 2-8 ℃
Note For Research Use Only.
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