• Talacotuzumab ELISA Kit
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AntibodySystem

KDD72501

Talacotuzumab ELISA Kit

Talacotuzumab ELISA Kit

Title

Catalog No.: KDD72501

Catalog No. KDD72501
Product name Talacotuzumab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human CD123 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Talacotuzumab in the sample competitively binds to the pre-coated protein with biotin-labeled Talacotuzumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Talacotuzumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Talacotuzumab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 46.90 ng/mL
Range 312.5 - 20,000 ng/mL
Recovery 80-120%
Background Talacotuzumab (TAL, JNJ-56022473) is an IgG1 monoclonal antibody targeting CD123 preferentially via antibody-dependent cellular cytotoxicity (ADCC) mediated by natural killer (NK) cells and has been shown to induce potent in vitro ADCC against IL3RA-expressing AML blasts/LSC and to reduce leukemic cell growth in murine xenograft models of human AML. In addition, the antibody inhibits signaling by IL-3, the main ligand of CD123, to reduce the proliferation of leukemic progenitor cells.
Alternative Names CSL-362-AML, CSL362, JNJ-56022473, CAS: 1826831-79-1
Shipping 2-8 ℃
Note For Research Use Only.
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