• Itolizumab ELISA Kit
1 1

AntibodySystem

KDD87601

Itolizumab ELISA Kit

Itolizumab ELISA Kit

Title

Catalog No.: KDD87601

Catalog No. KDD87601
Product name Itolizumab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human CD6 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Itolizumab in the sample competitively binds to the pre-coated protein with biotin-labeled Itolizumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Itolizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Itolizumab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 69.97 ng/mL
Range 234.38 - 15,000 ng/mL
Recovery 80-120%
Background Itolizumab, a humanized anti-CD6 monoclonal antibody, is a new molecule that acts by immunomodulating the CD6 molecule. CD6 is a co-stimulatory molecule required for optimal T-cell stimulation by the antigen-presenting cells. This step is crucial in T-cell proliferation to form Th1 and Th17 cells, which play a major role in the pathogenesis of psoriasis.
Alternative Names T1h, CAS: 1116433-11-4
Shipping 2-8 ℃
Note For Research Use Only.
전체 세부 정보 보기