• Ramucirumab ELISA Kit
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AntibodySystem

KDE16602

Ramucirumab ELISA Kit

Ramucirumab ELISA Kit

Title

Catalog No.: KDE16602

Catalog No. KDE16602
Product name Ramucirumab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human CD309 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Ramucirumab in the sample competitively binds to the pre-coated protein with biotin-labeled Ramucirumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Ramucirumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Ramucirumab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 80.76 ng/mL
Range 156.25 - 10,000 ng/mL
Recovery 80-120%
Background Ramucirumab is a human monoclonal antibody (IgG1) against vascular endothelial growth factor receptor 2 (VEGFR2), a type II transmembrane tyrosine kinase receptor expressed on endothelial cells. By binding to VEGFR2, Ramucirumab prevents binding of its ligands (VEGF-A, VEGF-C, and VEGF-D), thereby preventing VEGF-stimulated receptor phosphorylation and downstream ligand-induced proliferation, permeability, and migration of human endothelial cells.
Alternative Names 1121B, IMC-1121B, LY3009806, CAS: 15966-93-5
Shipping 2-8 ℃
Note For Research Use Only.
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