• Astegolimab ELISA Kit
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AntibodySystem

KDF88301

Astegolimab ELISA Kit

Astegolimab ELISA Kit

Title

Catalog No.: KDF88301

Catalog No. KDF88301
Product name Astegolimab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human IL1RL1 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Astegolimab in the sample competitively binds to the pre-coated protein with biotin-labeled Astegolimab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Astegolimab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Astegolimab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 32.28 ng/mL
Range 46.88 - 3,000 ng/mL
Recovery 80-120%
Background Astegolimab, a human IgG2mAb, selectively inhibits the IL-33 receptor, ST2. Approved biologic therapies for severe asthma mainly benefit patients with elevated blood eosinophils (type 2-high), but limited options are available for patients with low blood eosinophils (type 2-low).
Alternative Names AMG-282, MSTT-1041A, RG-6149, RO-7187807, CAS: 2173054-79-8
Shipping 2-8 ℃
Note For Research Use Only.
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