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SKU(재고 관리 코드):KDH02205
Sintilimab ELISA Kit
Sintilimab ELISA Kit
Catalog No.: KDH02205
| Catalog No. | KDH02205 |
|---|---|
| Product name | Sintilimab ELISA Kit |
| Size | 96 T |
| Description | PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human CD279 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Sintilimab in the sample competitively binds to the pre-coated protein with biotin-labeled Sintilimab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Sintilimab bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Applications | Used for the quantitative determination of Sintilimab concentration in serum and plasma. |
| Stability and Storage | When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃. |
| Detection method | Colorimetric |
| Precision | CV<20% |
| Sample type | Plasma, Serum |
| Assay type | Quantitative |
| Sensitivity | 110.56 ng/mL |
| Range | 234.38 - 15,000 ng/mL |
| Recovery | 80-120% |
| Background | Sintilimab is a monoclonal antibody against programmed cell death protein 1 (PD-1). It could block the interaction between PD-1 and its ligands and help the anti-tumor effect of T-cells to recover. Sintilimab is developed by Innovent Biologics and Eli Lilly and Company and has been approved to treat relapsed or refractory classical Hodgkin lymphoma in patients who have undergone two or more lines of systemic chemotherapy by the National Medical Products Administration of China. Recently, sintilimab has been reported in plenty of literature and shows satisfying anti-tumor effect. Meanwhile, there are some reports showing its side effects. Overall, sintilimab has similar anti-tumor effects and a better safety profile compared to nivolumab and pembrolizumab in Hodgkin lymphoma, natural killer/T cell lymphoma and advanced non-small cell lung cancer. In this review, we aim to briefly describe the mechanisms, pharmacological characteristics, anti-tumor effects, predictive parameters of efficacy and side effects of sintilimab, providing valuable information of sintilimab for decision-making in the treatment of tumors in the future. |
| Alternative Names | IBI-308, , CAS: 2072873-06-2 |
| Shipping | 2-8 ℃ |
| Note | For Research Use Only. |
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