• Elezanumab ELISA Kit
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AntibodySystem

KDJ37701

Elezanumab ELISA Kit

Elezanumab ELISA Kit

Title

Catalog No.: KDJ37701

Catalog No. KDJ37701
Product name Elezanumab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human RGMA has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Elezanumab in the sample competitively binds to the pre-coated protein with biotin-labeled Elezanumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Elezanumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Elezanumab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 223.20 ng/mL
Range 312.5 - 20,000 ng/mL
Recovery 80-120%
Background Elezanumab is a fully humanized monoclonal antibody directed against repulsive guidance molecule A (RGMa). Studies in patients with multiple sclerosis (MS) demonstrate RGMa upregulation, which inhibits axonal growth and myelination, oligodendroglial regeneration and functional recovery after trauma or inflammation. Elezanumab treatment promoted axon regeneration, neuroprotection, remyelination, and immune modulation in several MS-relevant preclinical models. Elezanumab was previously well-tolerated as a single dose to healthy volunteers.
Alternative Names ABT-555, AE12-1Y-QL (derived from parental mAb AE12-1 and AE12-1Y), CAS: 1791416-49-3
Shipping 2-8 ℃
Note For Research Use Only.
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