• Pacmilimab ELISA Kit
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AntibodySystem

KDJ70104

Pacmilimab ELISA Kit

Pacmilimab ELISA Kit

Title

Catalog No.: KDJ70104

Catalog No. KDJ70104
Product name Pacmilimab ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human CD274 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Pacmilimab in the sample competitively binds to the pre-coated protein with biotin-labeled Pacmilimab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Pacmilimab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Pacmilimab concentration in serum and plasma.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type Plasma, Serum
Assay type Quantitative
Sensitivity 390.71 ng/mL
Range 781.25 - 50,000 ng/mL
Recovery 80-120%
Background Pacmilimab (CX-072) is a Probody therapeutic directed against PD-L1 designed and developed by CytomX. Pacmilimab is engineered to be conditionally activated in the tumor microenvironment by tumor-associated proteases and to remain predominantly masked in the circulation. In preclinical studies, pacmilimab induced anti-tumor responses comparable to those of the parental anti-PD-L1 antibody and demonstrated reduced toxicity.
Alternative Names CX-072, CAS: 2145091-51-4
Shipping 2-8 ℃
Note For Research Use Only.
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