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AntibodySystem

SKU(재고 관리 코드):KGP13002

HEK293 Host Cell Proteins (HCP) ELISA Kit

HEK293 Host Cell Proteins (HCP) ELISA Kit

Title

Catalog No.: KGP13002

Catalog No. KGP13002
Product name HEK293 Host Cell Proteins (HCP) ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for HEK293 HCP has been pre-coated onto a microplate. Standards or samples and a biotin-labeled antibody specific for HEK293 HCP are pipetted into the wells, standards or HEK 293 HCP in the samples form immunocomplex with immobilized antibody and biotinylated anti-HEK 293 HCP antibody. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HEK293 HCP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of HEK293 HCP concentration in biological products derived from HEK 293 and HEK 293F cell lines.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<20%
Sample type HEK293 HCP impurities in samples.
Assay type Quantitative
Sensitivity 5 ng/ml
Range 10.24 - 1,000 ng/mL
Recovery 70%-130%
Alternative Names HEK293 Host Cell Proteins (HCP)
Shipping 2-8 ℃
Note For research use only.
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