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AntibodySystem

SKU(재고 관리 코드):KHC33701

Human sCD14 ELISA Kit

Human sCD14 ELISA Kit

Title

Catalog No.: KHC33701

Catalog No. KHC33701
Product name Human sCD14 ELISA Kit
Size 96 T
Description PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human sCD14 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human sCD14 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Human sCD14 is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human sCD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications Used for the quantitative determination of Human sCD14 concentration in serum, plasma and cell culture supernatant.
Stability and Storage When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method Colorimetric
Precision CV<15%
Sample type Serum, Plasma and Cell culture supernatant.
Assay type Quantitative
Sensitivity 27.26 pg/mL
Range 93.75 - 6,000 pg/mL
Recovery 80-120%
Alternative Names Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14
Shipping 2-8 ℃
Note For Research Use Only.
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