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AntibodySystem

SKU(재고 관리 코드):YVV07701

3C Protease

3C Protease

Catalog No.: YVV07701

3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.

Catalog No. YVV07701
Product name 3C Protease
Size 5,000 U
Expression system E.coli
Protein length 3C Protease is cloned from Human rhinovirus and expressed in E.coli.
Predicted molecular weight 47.46 kDa
Description 3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Form Liquid
Storage buffer 50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol
Purity >95% as determined by SDS-PAGE.
Applications Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Biological activity One unit will cleave ≥95% of 100μg of GST-tagged protein in 16 hours at 4°C.
Endotoxin level Please contact with the lab for this information.
Nature Recombinant
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Species HRV-14
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Note For research use only.
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